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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has become observed in many tumors, which leads to chromosome instability, and so may well contribute to tumorigenesis. This protein was discovered to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical purpose in selling cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, have already been described. Two added splice variants had been noted but comprehensive nucleotide sequence information and facts is just not nonetheless obtainable.

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Blocking peptides are peptides that bind specially to the target antibody and block antibody binding. These peptide commonly is made up of the epitope regarded from the antibody.

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This tutorial outlines further feasible optimization of cellular and immunoassay parameters to be certain the very best outcomes are...

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This information outlines even more doable optimization of mobile and immunoassay parameters to be certain the very best success are...

Antibodies sure to the blocking peptide now not bind into the epitope over the concentrate on protein. By evaluating the staining within the blocked antibody vs . the antibody by itself, you can see which staining is distinct.

Goal Details Cyclin E belongs for the extremely conserved cyclin family members, whose members are characterised by a spectacular periodicity in protein abundance in the cell cycle. Cyclins purpose as regulators of CDK kinases. Unique cyclins show distinctive expression and degradation designs which contribute into the temporal coordination of every mitotic occasion. Cyclin E forms a posh with and features as a regulatory subunit of CDK2, whose exercise is required for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and it is degraded as cells development by way of S phase.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...

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Clicking the photographs or links will redirect you to an internet site hosted by BenchSci that provides 3rd-occasion scientific content. Neither the content material nor the BenchSci technological innovation and procedures for collection are actually evaluated by us; we are supplying them as-is and with out warranty of any type, which includes for use or application from the Thermo Fisher Scientific items presented.

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